Additional analysis if no inferences are realistic from tables 3 and 4, attempt deeper analysis of the mass spectrum. Mass spectral interpretation is the method employed to identify the chemical formula, characteristic fragment patterns and possible fragment ions from the mass. Mclafferty rearrangement is the most important fragmentation reaction observed in the mass spectrometry. Mclaffertys most popular book is interpretation of mass spectra. Cho mz 44 is produced by the mclafferty rearrangement and c h mz 56 is the. The chapter includes an introduction to the main ionisation techniques in mass spectrometry and the way the resulting fragments can be analysed. First, the fundamental notions of mass spectrometry are explained, so that the reader can easily cover this chapter graphs, main pick, molecular ion, illogical pick, nitrogen rule, etc. Interpretation of msms mass spectra of drugs and pesticides wilfried m.
Mclafferty sooner you get guide interpretation of mass spectra, by fred w. Massspectrograms in some ways are easier to interpret because information is. Indeed, mass spectrometry appears to be regarded as a mysterious method, just good enough to supply some molecular weight information. It will be your resort to maintain downloading guide interpretation of mass spectra, by fred w. Shows you how to get the information out of a mass spectrum and use it to help suggest the identity of an unidentified molecule. Isotopic percentage and nominal mass calculation are also. These were sorted and checked at cornell by the mclafferty group, with approximately 4000 additional corrections made there. Interpretation of mass spectra by mclafferty, fred w. Chapter 2 fragmentation and interpretation of spectra 2. Before discussing fragmentation and interpretation, it is important to. Turecek published by university science books, mill valley, 1993 isbn 0.
Schematics of an electrospray source where ions separate by the m z ratio z. Pdf interpretation of msms mass spectra of drugs and. In mass spectrometry, one generates ions from a sample to be analyzed. Examine spectrum for peak clusters of characteristic isotopic patterns. Mass spectrograms in some ways are easier to interpret because information is. Media in category mass spectra interpretation the following 21 files are in this category, out of 21 total. Definitions of terms relating to mass spectrometry iupac. If good inferences could be made, use the remainder of the mass spectrum to try to elucidate structural features or isomerism. For the analytical chemist, a mass spectrum is useful for two applications. The leading scientist in this field was fred mclafferty who together with frantisek turecek has written a very famous text book.
While ketones undergo a single mclafferty rearrangement. Mclafferty has 16 books on goodreads with 26 ratings. Automated matching and interpretation of unknown mass spectra. When nonmass spectrometrists are talking about mass spectrometry it rather often sounds as if they were telling a story out of poes tales of mystery and imagination. Lecture notes in eimass spectrometry by torben lund. Gas chromatographymass spectrometry gc ms interpretation of ei spectra jeremy keirsey ccic msp. Before discussing fragmentation and interpretation, it is important to understand the many ways mass spectra are utilized. Extensively revised and updated, this new edition, as in pr. An introduction to mass spectrometry these pages have moved. Fast atom bombardment and secondary ion mass spectrometry. A basic approach, second edition combines coverage of the principles underlying mass spectral analysis with clear guidelines on how to apply them in a laboratory setting. Unknown identification using reference mass spectra. Which requires at least one gamma hydrogen and one site for radical cation generation.
1127 1484 1026 182 332 684 561 336 647 315 480 1373 1210 348 441 786 1222 1123 51 278 143 307 739 532 658 289 1309 802 415 252 508 88 825 1347 661 1128 37 527 1400 1098